General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GRPR Activated GPCR Internalization Assay, 10pl Kit. (93-0667E3CP5L)
The PathHunter GRPR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GRPR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR107 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0419E2ACP1L)
The PathHunter GPR107 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR107 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR107 and EA-tagged β-Arrestin-2. Activation of GPR107 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, HEK293, 2pl Kit. (93-1001E1CP0M)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc DLX3 Transcription Factor Activity Assay
DLX3 Transcription Factor Activity Assay
Cayman Chemical Mitochondrial Complex III Activity Assay Buffer, 10 mL
Mitochondrial Complex III Activity Assay Buffer, 10 mL
American Research Products Inc Human cAMP response element bi
Human cAMP response element-binding protein (CREB) ELISA Kit from CUSABIO Detection Range 0 312 ng/mL-20 ng/mL Sensitivity 0 078 ng/mL Sample serum plasma tissue homogenates Method Sandwich
American Research Products Inc TBX1 Transcription Factor Activity Assay
TBX1 Transcription Factor Activity Assay
Eurofins Discoverx AssayComplete Cell Plating 28 Reagent, 100. (93-0563R28A)
AssayComplete Cell Plating 28 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical Nitrate/Nitrite Colorimetric Assay Kit (LDH method), 96 Well
NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a NOS-catalyzed reaction, thereby making the assay particularly well suited to measurements of NOS activity in vitro. High throughput screening applications using recombinant NOS preparations can be quickly and accurately assayed with the LDH method kit.
Eurofins Discoverx PathHunter eXpress TrkC Functional Assay, 2-Plate Kit. (93-0464E3CP16M)
The PathHunter eXpress TrkC Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DRD5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0426E2CP0L)
The PathHunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD5 and EA-tagged β-Arrestin-2. Activation of DRD5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0569
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0569
American Research Products Inc TBX2 Transcription Factor Activity Assay
TBX2 Transcription Factor Activity Assay
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 2pl Kit. (93-1009E3CP16M)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.